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Registros recuperados : 28 | |
1. | | MENCHACA, A. Assisted Reproductive Technologies (ART) and genome editing to support a sustainable livestock. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230074. http://dx.doi.org/10.1590/1984-3143-AR2023-0074 -- OPEN ACCESS. Article history: Received May 26, 2023; Accepted July 21, 2023. -- Document type: Article Gold Open Access, Green Open Access. -- Correspondence: Menchaca, A.; Plataforma de Salud Animal, Instituto Nacional de Investigación...Biblioteca(s): INIA Las Brujas. |
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3. | | MENCHACA, A. Sustainable food production: The contribution of genome editing in livestock. Sustainability (Switzerland), June 2021, volume 13, Issue 122, article 6788. OPEN ACCESS. Doi: https://doi.org/10.3390/su13126788 Article history: Received 16 March 2021; Revised 31 May 2021; Accepted 1 June 2021; Published 16 June 2021.
Academic Editor: Gerhart U. Ryffel.
This article belongs to the Special Issue Genome Editing in Animal System to Support...Biblioteca(s): INIA Las Brujas. |
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4. | | BÓ, G.A.; MENCHACA, A. Prohibition of hormones in animal reproduction: what to expect and what to do?. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230067. https://doi.org/10.1590/1984-3143-AR2023-0067 -- OPEN ACCESS. Article history: Received May 15, 2023; Accepted: July 18, 2023. -- Financial support: GAB received funding for this research from Fondo Nacional de Ciencia y Tecnología (FONCYT PICT 2017-4550), Instituto de Investigación Universidad...Biblioteca(s): INIA Las Brujas. |
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8. | | GARCÍA PINTOS, C.; RIET-CORREA, F.; MENCHACA, A. Effect of foot-and-mouth disease vaccine on pregnancy failure in beef cows. Frontiers in Veterinary Science, November 2021, volume 8, Article number 761304. Open Access. doi: https://doi.org/10.3389/fvets.2021.761304 Article history: Received 19 August 2021; Accepted 18 October 2021; Published 12 November 2021.
Corresponding author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay;...Biblioteca(s): INIA Las Brujas. |
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13. | | KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. Chapter 16. Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16Biblioteca(s): INIA La Estanzuela. |
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15. | | NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United...Biblioteca(s): INIA Las Brujas. |
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19. | | DOS SANTOS-NETO, P.C.; CUADRO, F.; SOUZA-NEVES, M.; CRISPO, M.; MENCHACA, A. Refinements in embryo manipulation applied to CRISPR technology in livestock. Theriogenology. 2023, Volume 208, Pages 142-148. https://doi.org/10.1016/j.theriogenology.2023.05.028 Article history: Received 14 April 2023; Received in revised form 29 May 2023; Accepted 29 May 2023; Available online 9 June 2023. -- Correspondence author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy,...Biblioteca(s): INIA Las Brujas. |
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20. | | CUADRO, F.; DE BRUN, V.; BROCHADO, C.; SOUZA, M.; GARCÍA PINTOS, C.; MENEZES, C.; GASTAL, G.D.A.; MENCHACA, A. Gene expression patterns in uterus and oviduct during the preovulatory period in ewes. In: 19th International Congress on Animal Reproduction (ICAR), Bologna (Italy), 26-30 June 2022. p.185.Biblioteca(s): INIA La Estanzuela. |
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Registros recuperados : 28 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
24/10/2023 |
Actualizado : |
24/10/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
DOI : |
10.1111/imb.12875 |
Idioma : |
Inglés |
Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
Asunto categoría : |
L01 Ganadería |
URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
|
Marc : |
LEADER 02928naa a2200313 a 4500 001 1064356 005 2023-10-24 008 2023 bl uuuu u00u1 u #d 022 $a0962-1075 (print); 1365-2583 (electronic). 024 7 $a10.1111/imb.12875$2DOI 100 1 $aNOVAS, R. 245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- 520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aPartnership for the goals - Goal 17 653 $aPest control 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aScrewworm 653 $aSustainable Development Goals (SDGs) 653 $aU6 promoter 700 1 $aBASIKA, T. 700 1 $aWILLIAMSON, M. E. 700 1 $aFRESIA, P. 700 1 $aMENCHACA, A. 700 1 $aSCOTT, M. J. 773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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